The
MICROBIOLOGY 102 WEBSITE
for
SPRING SEMESTER, 2013

x

x

Links
There are more course-related links to come,
and we will eventually get to each one.

UW-Madison Academic Calendar
UW-Madison Schedule of Classes

xGENERAL COURSE-RELATED ITEMS:
Syllabus.
Revised Schedule.  (Yes, we always start on
   the first week of the semester!)
Some frequently-asked-about policies:
  ·Getting your two credits-worth.
  ·Attendance policy.
  ·Bad weather policy.  (Also see the weather
     link at the bottom of the page.)
Old Quiz Questions (Exps. 1-7)
Old Quiz Questions (Exps. 1-7) – Key
Old Quiz Questions (Exps. 8-17)
Old Quiz Questions (Exps. 8-17) – Key
Sample Final Exam Questions
Sample Final Exam Questions – Key
Take-Home Exercise No. 1
Take-Home Exercise No. 2
Take-Home Exercise No. 3

xSOME IMPORTANT HANDOUTS:
Microscope Handout – Page:  1, 2.
Lab Manual Pages – Period:  1, 2.
General Catabolism & Oxygen Relationships
Catabolism: Chemotrophy vs. Phototrophy
Oxygen Relationships Summary
Growth Curves: Class Data

xLAB LECTURE NOTES:
   [Usually posted ahead of time. *=a "rough draft."]
Period  1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,
   13 and 14.

xTHE COMPLETE VIRTUAL LABS:
      [Complete list for the Semester.]
Introduction to the Virtual Lab Experience
Virtual Experiment 3B (Scroll to "Period 4.")
Virtual Experiment 4B
Virtual Experiment 5A
Virtual Experiment 5B: Making Media and
   Growth Factor Requirement (siderophores)
Virtual Experiment 6. This is supplemented
   by virtual demonstrations showing flagellar
   and gliding motility. (The latter shows a
   gliding mass of cells.)
Virtual Experiment 7B. This is basically a
   virtual demonstration which will be supple-
   mented by a brief explanation in lab.  (An
   older, more detailed version is given here.)
Virtual Experiment 8B: Mutation and
   Conjugation/Recombination (quantitative)
   The solutions are posted here.
Virtual Experiment 9B
Virtual Experiment 10 (A handout will be pro-
   vided in lab to replace the on-line images,
   and a helpful diagram can be found here.)
Virtual Experiment 11D
Virtual Experiment 12
Virtual Experiment 13
Virtual Experiment 14B
Virtual Experiment 15B which deals with the
   MPN Method of enumerating bacteria.
   The solutions are posted here.
Virtual Experiment 16 which deals with
   Koch's Postulates (p. 81).

xBASIC METABOLISM & MEDIA LINKS:
Catabolism & Oxygen Relationships:
  ·Introductory Handout
  ·Chemotrophy vs. Phototrophy
  ·Oxygen Relationships Summary
Differential Test Media (mainly pH-based):
  ·Our DIFFERENTIAL MEDIA WEBSITE
     includes a number of media which we use
     such as Motility Medium, Starch Agar and
     Glucose Fermentation Broth. Also note
     the General Overview.
  ·Whiteboard overview (with "O2 relationships")
  ·More about Glucose Fermentation Broth:
     Usual whiteboard view and a comparison
     to Glucose O/F Medium.
  ·Reactions in Modified MacConkey Agar
  ·Reactions in KIA (plus usual whiteboard view
     and errors in inoculation & incubation).
  ·Decarboxylation Test Media
  ·Enteric Test Reactions (other than KIA)

xGENERAL CONCEPTS:
      [Complete list for the Semester.]
Aseptic Technique:
  ·General list of procedures
  ·Tube-to-tube transfers with video
  ·Three-phase plate-streaking with video
CFU, Colony, Isolate and Strain:
  ·Definition of colony-forming unit (CFU)
  ·From CFU to colony
  ·Definitions of isolate and strain
Mutation and Recombination (Exp. 8):
  ·Our old handout – HTML and PDF
Bacterial Quantitation:
  ·Dilution Plating: Pages 1, 2
  ·Most Probable Number (MPN) Method
Bacterial Identification:
  ·Phenotypic
  ·Genotypic
Enrichment & Isolation of Bacteria (Exp.11):
  ·Our old Exp. 11 supplementary worksheet
  ·Diagram of endosporeformer isolation. Close-
     up of colony development is shown here.
  ·Exploitable characteristics of certain bacteria
Coliforms (enrichment, detection & isolation):
  ·Basic facts
  ·Procedural flow chart
Enterics:
  ·Basic facts and isolation
Dichotomous Keys:
  ·Flow Charts, Tables and Dichotomous Keys
  ·Handout with an example

Course Announcements
(newest updates first)


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Monday, May 20, 2012

Every semester about this time I get inquiries (all very similar, like a form letter) about the possiblity of doing extra credit with the hopes of a higher grade resulting from that. I can never go along with that. For one thing, it would not be part of the grading scheme indicated in the syllabus. Also – to make things fair and ethical – I would have to make the opportunity known much earlier to everyone if I did agree with that idea. As it is, I do tend to ease up a little on the final grading scale, and AB's and B's are still very good, respectable grades.

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Wednesday, May 15, 2013
Report Scores Posted

In grading the reports which took awhile and was somewhat subjective, I assigned a letter grade along with some comments on each report. The Bacillus reports got the most comments. I eased up on the "grading scale" (for the reports) and gave points as follows: A=60, AB=58, B=56, BC=54, C=52.

When you check your report grade and see a blank or zero, that means I have not received it via the drop box or otherwise. (I assume everyone has their own copy of the report and can run off a copy if necessary.) I will still accept late stuff through early Friday afternoon.

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Sunday May 12, 2013
Evaluations, Reports and Coliform Clarification

In case you're worried about confidentiality of your evaluations, I don't ever get to associate any evaluation with any individual. I only get to see the overall summaries. However, I will be able to find out who filled out a report such that bonus points can be given.

The Experiment 11 Reports are very slow-going, and I am still hoping to have them done by exam time Wednesday as I mentioned last week. As I had announced that doing a report on Exp. 15A was OK as an alternative (if any of the parts of Exp. 11 didn't turn out all that well), I've finished grading those but I'd like to go back and re-read them.

Remember the definition of coliforms:

  • These are strains that share the characteristics of being gram-negative rods and also fermenting lactose with the production of acid and gas.
  • You may have seen an extended definition which states that they ferment lactose (with visible gas production) in Lactose Lauryl Tryptose Broth (LLTB) and also Brilliant Green Lactose Bile (BGLB) Broth. These media are important in the Presumptive Tests where we are selectively enriching for them and also detecting their presence among the mixed cultures in these media; these media are selective against Gram-positive bacteria, especially BGLB.
  • We could have used LLTB and BGLB in the Completed Tests when we tested the isolates we obtained off EMB Agar, but we simply tested for acid and gas production in Lactose Fermentation Broth. (And instead of doing the gram stain, we assumed that growth on EMB Agar was indicative of being gram-negative.)
  • You may have seen in some of the literature that coliforms are "aerobic or facultatively anaerobic." This is an old-school description of what we call facultative anaerobes. They do grow as strict aerobes unless we give them an opportunity to grow under anaerobic conditions – such as fermentation. As they all ferment lactose, they will all ferment glucose as well. (If any organism ferments a sugar, it is assumed they can ferment at least glucose.)

Also remember that coliforms are not the problem but rather an easily-detectable type of organism that is indicative of certain problems:

  • If a coliform is identified as Escherichia coli, that means the environment was contaminated with fecal matter. It's a lot easier to look for E. coli than any of the other intestinal pathogens which may be bacterial, protozoan or viral!
  • Remember we mentioned (and it's often forgotten) that well water is tested for coliforms. Many coliforms are found growing naturally in soil, and if they are detected in drinking water, that means that surface soil runoff is getting into the well, bringing problems that affect the water's safety.
  • It's true that certain types of E. coli and Klebsiella happen to be pathogenic, but our emphasis was on the use of coliforms as indicator organisms of problems associated with the environment in which they are found.
  • As "coliform" is not a taxonomic group, you can expect that the various species of Escherichia, Klebsiella, etc. can have lactose-negative strains, in which case those strains would not be called "coliforms."

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Thursday, May 9, 2013
The three catabolism-related tests in Experiment 17.

If you are still not 100% certain what is ultimately being tested for in the three media in Experiment 17A, check the Lab Lecture Notes for Period 14 which are posted here. There is no reason why an organism cannot be able to have the ability to be positive for all three tests. For example, the enterics are all capable of aerobic respiration, anaerobic respiration and fermentation as shown on the Experiment 14 Handout.

Remember how bacteriologists define strict aerobes and facultative anaerobes in Experiment 5A, and this carried over into Exp. 7A and 11C. The only catabolic processes that are relevant in "oxygen relationships" are aerobic respiration and fermentation. See the table on page 24.

If a strict aerobe is also able to use nitrate as an electron acceptor in place of oxygen, this is anaerobic respiration, and the organism is thus able to grow anaerobically if nitrate is present. The organism is still a strict aerobe! Anaerobic respiration and also phototrophy are "special" catabolic processes and do not affect an organism's oxygen relationship designation.

If you see something like this in Glucose Fermentation Broth, remember that any yellow color means acid is produced from fermentation. (And a fermenter does not have to produce gas!) That purple color on top is just an alkaline reaction from aerobic amino acid deamination that has not been overneutralized by acid from the fermentation of glucose; if it were overneutralized by the acid, then we would have a completely yellow tube. This was gone over in Exp. 7A, and the influence of the alkaline reaction from amino acid deamination is critical in how KIA works (Exp. 14A).

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Tuesday, May 7, 2013
Posting Scores on Learn@UW – UPDATED

I have posted the scores at Learn@UW for everything except the Experiment 17 checklist, reports and final exam. Please let me know if anything is missing or wrongly recorded on my part.

A reminder that I will be gone Thursday evening until Sunday evening, and I will still be emailable during that time.

Reports will take awhile to go through and they should be ready to pick up by the officially scheduled final exam time. I'm following a checklist which includes a lot of items on page 128.

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Tuesday, May 7, 2013
Evaluating the Course – UPDATED

Stay tuned for an e-mail message from the Department about where to access the course evaluation. You can fill out the evaluation checklist for Microbiology 102 from late Thursday, May 9 through Friday, May 17.

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Friday, May 3, 2013
Reports, Exam and Evaluations

If you're in a pinch and can't drop off the report, I'll accept it via e-mail. (As mentioned below, what I don't like is reviewing rough drafts by e-mail as I must do such a thing personally.) I can't start grading them until late Sunday or Monday.

Following is a list of hints that can help in reviewing for the final (not necessarily in any particular order):

  • Links to the old quiz questions and sample final exam are in the links box on the right. These are great for review, and they show that we don't ask about really trivial things (like differences between species, and color reactions). Answers are posted for all.

  • You can go through the procedures in the manual, asking yourself "what did I do and why?" and especially noting the things in bold face.

  • Appendices that are good to review are B, C, D (especially the bold-faced terms), F and G. There are some good summaries of various media we used in Appendix E, but don't memorize the ingredients!

  • Also good are the introductions to the various experiments, especially Exps. 1 and 11C. It can be surprising to find that the introduction to Exp. 1 makes a lot more sense after your experience this semester, and the same goes for Appendix D.

  • Remember the big deal we occasionally made about catabolic processes and also the reasons for pH changes in differential media. Understanding the table on page 22 in the Exp. 5A introduction and also the KIA question on the third take-home exercise can help considerably. (Don't memorize what specific organisms do in these items, however.)

  • Don't memorize the colors of test reactions or what specific species do (such as in the identification tables for Experiments 7A and 14A). We did emphasize characteristics of certain groups (purple non-sulfur photosynthetic bacteria, enterics, and coliforms) and certain genera (the characteristics of Bacillus and Streptomyces and how each can produce a distinct type of cell besides the vegetative cell). As mentioned in a previous update, the characteristics of certain types of bacteria which led to our use of specific procedures and media to isolate them is here.

  • If the terms genus and species are still confusing, read over Appendix H which includes some of the things that one is expected to retain from general biology courses. A strain is nothing more than an isolate that you know something about; never substitute "strain" for genus or species. Also never use media and bacteria as singular terms (which would be medium and bacterium). This is also pointed out in Appendix I in the guidelines for the lab reports!

  • Be sure you can differentiate:
    • isolate (the verb) vs. isolate (the noun)
    • enrichment vs. isolation
    • conjugation vs. recombination
    • mutation vs. recombination
    • cell vs. CFU vs. colony
    • phage vs. PFU vs. plaque
    • selective vs. differential media

  • And always remember that when you define a term:
    • Do not add a description (i.e., additional, irrelevant details) of an organism that goes along with that term.
    • The definition should not be so broad as to include more than what the definition indicates. As an example, heterotroph refers to the kind of thing that is used as the carbon source, not as the source of reducing power (electron donor). And watch how you define antibiotic and enrichment! These terms are gone over in Experiments 10 and 11, and never confuse antibiotics with antibodies!
    • Examples and analogies really don't help much. These things along with excess specificity can really ruin a good definition.
    • Define the term according to how it is applied in this course!

  • Keep the answers to "short answer" questions short. Itemize when you can. Please do not repeat the question as part of the answer.

Finally, we are required to provide the opportunity for you to evaluate the course on-line. Bonus points will be added to your total points score for doing so. Details soon.

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Wednesday, May 1, 2013
Times and Places for Final Exam – Official and Alternate

Official:  Wednesday, May 15 at 12:25 PM in 1125 Biochemistry.
Alternate Times are all given in the lab:
   Monday, May 13 at 10:05 and 12:25.
   Tuesday, May 14 at 10:05.
   Thursday, May 16 at 12:25.

There is a sign-up sheet in the lab for the alternate times which you are not required to sign. I understand you have a good reason for taking the exam at the alternate time, so I need not keep a record of such a thing. You don't have to e-mail me about this, and you can switch times as necessary.

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Tuesday, April 30, 2013
Quoting Questionable Sources and the Definition of "Isolate"

Despite the fact we were signing our names to put the answers in our own words on the third take-home exercise, I saw all-too-many repetitions of the same phrase when it came to the definition of isolate (as a noun). Repeated, copied statements stand out like a sore thumb to a seasoned instructor – especially when reading reports – and most often they are too general or naive to apply to what we are doing in lab. So, when googling the phrase "individual, population, strain, or culture," Google informed me of "about 76 results" – right down to the unusual indication of a comma before "or." And it was generally followed by "obtained by or resulting from selection or separation." Just putting these quotes for the definition is not enough for a microbiology lab course. An isolate is simply a pure culture derived from a sample containing a mixed population of microorganisms, and this can be accomplished by streak-plating and dilution plating (which includes the use of spread and pour plates). You could probably come up with a better definition.

An "A" report would not resort to such repetition without an express reference to the source which can then be evaluated according to relevance to the course. By the way, when I read reports, I often check on the references and happily find out things (from reputable sources) that I never knew before. [insert smiley face here]

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Monday, April 29, 2013
Old Exam Questions and "Exploitable Characteristics"

Questions from an old final exam are posted here. Answers will be provided later such that you can compare your answers with them.

Regarding the characteristics of the organisms we were isolating in Experiments 11(A, B and C) and 15A which we made use of in the isolation process:  Keep in mind that the lab report guidelines (Appendix I) require that these characteristics be included in the introduction to the report. As a reminder, there is a good summary here, based on the introductions to the specific experiments. Gram reaction is not always relevant, and we did not need to do gram stains when isolating Bacillus, Streptomyces, or the photosynthetic bacteria. (Exp. 14A is included in this summary, although that experiment is not considered for a report.)

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Thursday, April 25, 2013
Some More Lab Report Information

About referencing the lab manual:  For that, as well as other things in book form, you would want to indicate author (or editor), title, year and publisher. Depending on the style of referencing, you would need to put these items in the appropriate order. One way of referencing the manual could be like this:
Lindquist, John (ed.). 2013. Microbiology 102 – General Microbiology Manual for Spring Semester. University of Wisconsin, Madison.

By all means, do not have the manual as your only reference! Altogether, at least three would be nice, but two should be the minimum.

Also, feel free to use the table formatting that is in the manual. There is no restriction on that. In fact, if you can improve on it, please do so. Don't forget that the footnotes (under the tables) in the manual are part of the tables.

In case you discarded the lab handout for Period 11, here are the four test organisms (in no particular order):
BS = Bacillus subtilis
PF = Pseudomonas fluorescens
EC = Escherichia coli
EF = Enterococcus faecalis

If you would like to have me briefly look over a rough draft, I can do so but not by e-mail. It would be a lot more instructive and less time-consuming to do that personally.

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Thursday, April 25, 2013
Lab Report Questions & Answers

Answers to some frequently-asked questions follow. Most have already been mentioned in lab, and remember to follow the Guidelines in Appendix I.

  • Not to worry that the Lab Report has to be turned in by lab time. You can turn it in to our drop box any time next week until the building locks up at 5PM on Friday, May 3.

  • The Guidelines in Appendix I are indeed correct about the necessary shortness of the Materials and Methods section! Keep it short. When I went over this in lab and suggested looking at published scientific papers (Steenbock is full of them), you will see that a lot of methods are referred to (in other papers) and are not spelled out in further detail unless modifications were made. Having one or two pages devoted to a reiteration of the procedures we did in lab will be a waste of time and certainly will not help the grade of the report.

  • There is a lot of information in the introductions to the experiments! Don't depend on Wikipedia for things. For example, page 107 is referred to in the introduction to Experiment 11A for an explanation about the medium used to isolate Streptomyces. And the introduction to Exp. 11C is basically a summary of soil microbiology.

  • How long should it be? There is no page limit. Most turned in over the years have been 4-6 pages.

  • So, the report can be on any part of Experiment 11 – i.e., 11A, 11B or 11C. As mentioned in lab, you can do it on Experiment 15A if the others aren't working out satisfactorily. You can title it Enrichment and Isolation of Coliforms as it does indeed follow the principles of enrichment and isolation summarized in the introduction to Exp. 11. Exp. 14A would not work, as it's an unknown exercise not utilizing natural sources (soil, water, etc.) nor involving the concepts of Exp. 11.

  • Forgot where a sample came from? Look under the April 2 update, below.

  • And keep things as simple and organized as possible.

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Monday, April 22, 2013

I'll be in very late today – probably after 5PM or so. (That means a late-nighter for me!) Presently I'm up north, having finished some blizzard-related chores and will be hitting the road soon.

Read that box under your name on the Take-Home No. 3 – especially regarding the due date and where to submit it. The bonus question is meant as a thought question, solved through the process of elimination. Should be fun and/or easy.

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Friday, April 19, 2013
Posting Grades on Learn@UW

I will soon be posting the scores of the various quizzes, unknowns, etc. we've done so far this semester, such that you can compare your records to mine and I can make any corrections accordingly.

The question often comes up regarding how are we doing grade-wise so far. If you're interested in what letter grade corresponds to your overall score so far, simply add up your scores and divide them by the total number of points those items were worth. These items are all listed on the bottom of the 2nd page of the Syllabus along with the percentage ranges associated with the letter grades.

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Thursday, April 18, 2013
Take-Home Set No. 3

The Take-Home Exercise No. 3 is now posted here. Note that it can be handed in any time next week, and it should be put in the Microbiology 102 drop box which is the top hallway locker, right next to the front door of the lab. It is labeled as such, and you can't miss it!

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Monday, April 15, 2013
Solution to Virtual Experiment 15B

The solutions to the plating and MPN problems in Virtual Experiment 15B are posted here.

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Thursday, April 11, 2013
More about lab reports. Some on-line demonstrations. Also a sample to get?

The Lab Report is due in three weeks and it can be handed in any time that week (through 5 PM Friday, May 3) to the drop box in the hallway.

  • Format and other guidelines are listed in the last Appendix (pages 125-128) of the Manual. Note the strong recommendation to look at some published papers in scientific journals; Steenbock Library many bound volumes full of them. This is not a requirement that you have to use these journal papers in your report, but you can use them as examples of how they follow an overall format and how figures and tables are handled. You won't be required to include figures or an Abstract. You can use the same tables of results that you've filled out in the manual, and feel free to improve on the format as you like. Let me know if you have questions.

  • The referencing guidelines from the Helen C. White Library Writing Lab (mentioned in Appendix I and possibly useful for other courses as well) are downloadable on a pdf file here. For referencing, you can use whatever referencing format you are used to, as long as it's applied consistently.

  • If you are still confused about genus and species, re-read Appendix H. In fact, an expanded and clarified version is on-line here. It is too bad that biology courses don't effectively teach about taxonomy and nomenclature any more.

Following are a few notes about on-line demonstrations associated with Experiments 14A and 15A:

  • A virtual demonstration of selective-differential media for enterics is shown here.

  • The membrane filtration method mentioned in Experiment 15A is basically where a water sample is run through a filter, and the filter is plated on a medium appropriate for the detection and isolation of a certain type of bacterium.
    • An example: Twenty five ml of a water sample were passed through a filter with the bacteria being trapped on the surface of the filter. The filter was then placed on Experiment 14's Modified MacConkey Agar (which has indicators for lactose fermentation and hydrogen sulfide production). After 24 hours at 37°C, the black colonies seen here are of H2S+ bacteria whose colonies can then be counted and isolated.
    • Likewise, fecal coliforms can be detected as lactose-fermenting colonies on a filter which was placed on a medium such as regular MacConkey Agar which was then incubated for 24 hours at 44.5°C (the incubation temperature we used for EC Broth).

  • And the API-20E rapid method for enteric identification is shown here.

Finally, you might like to bring in a sample to plate out for Experiment 17A which starts next week. Check Period 1.

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Thursday, April 4, 2013
A couple notes about Virtual Experiment 10 and Exp. 11C.

Virtual Experiment 10 (which is to be viewed along with the handout passed out in lab):  A helpful diagram related to our discussion of the Antibiotic Disk Sensitivity Test can be found here. If the culture contained some cells that had gained resistance to Antibiotic A by mutation, those cells are indicated in red, and their colonies would show up in the "zone of inhibition" around the Antibiotic A disk. Clearly, Antibiotic D would be the antibiotic of choice if the diameter of the zone of inhibition around that disk is a certain minimum size (according to a table such as what is found on the handout).

Experiment 11C:  Remember the importance of the catalase test and Glucose Fermentation Broth when determining if any of our Bacillus isolates are strict aerobes or facultative anaerobes.

  • A positive catalase reaction indicates that the organism is capable of aerobic respiration. A positive indication of glucose fermentation indicates that the organism is indeed capable of fermentation which allows for anaerobic growth (as fermentation does not require the presence of O2 as does aerobic respiration.)

  • Recalling what we learned from Virtual Experiment 5A (page 22), an organism that only aerobically respires is a strict aerobe, and one that can aerobically respire and ferment is a facultative anaerobe. Any given species of Bacillus is one or the other.

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Thursday, April 4, 2013
A few things to help with Quiz 2 and the Lab Report.

Going along with the two lab lectures prior to Spring Break, what may not show up completely in the posted notes is a summary of characteristics of the various bacteria which we take advantage of when considering such things as media and incubation conditions for their isolation.

On our old and "retired" Bacteriology 102 website are some pages dealing with subject matter that are still kept reasonably up-to-date. Specific items that may be relevant and applicable to us these days are:

  • Purple non-sulfur photosynthetic bacteria. Included (at the bottom) is the "light vs. dark test" which we will be observing next week (with with our isolates). Note why it is not an "oxygen relationship test"!
  • The isolation of Bacillus.
  • An old lab report that has some comments made by the grader. Note that the writer of the report took sample collection seriously and went ahead and collected three samples instead of one. Hardly anyone brought in a sample for anything this semester. (We will require it this coming summer session.)

If you choose to do your lab report on Streptomyces (Experiment 11A) and are looking for references, there is one at Microbiology Bytes that is very popular but not well-written. It mentions that extracellular enzymes are commonly produced by Streptomyces which is correct. However, these enzymes are for breaking down molecules much larger than the compounds that are listed! Streptomyces uses extracellular enzymes to break down large macromolecules like cellulose, starch, chitin, complex proteins, and other such compounds in the process of biodegradation. Recall the concept of amylase in Experiment 7 and how an organism cannot utilize starch (as an energy source or for biosynthesis) unless it is broken down outside of the cell by extracellular enzymes. You can find much better Streptomyces references elsewhere.

Use Wikipedia and encyclopedias with extreme caution. They are most valuable in indicating references that are most likely very reliable.

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Wednesday, April 3, 2013
A correction about the quiz plus Virtual Experiment 5A again.

I erred below and in lab yesterday about what experiments are going to be on the quiz. Experiment 9 will not be included.

In correcting the Take-Home No. 2, I see a significant amount of misunderstanding about "oxygen relationships." At your convenience, you should review pages 20-23 in the manual – specifically Parts I, II, III and IV. It's all important stuff throughout the semester as it was when Virtual Experiment 5A was current.

  • When dealing with the usual, easy to grow, chemoheterotrophic organisms one generally encounters in a teaching lab (also a clinical lab, and other diagnostic laboratories), the oxygen relationship categories are good to have, as each one conveniently tells us three things about the organism: (1) whether or not it tolerates air, (2) whether or not it can aerobically respire, and (3) whether or not it can ferment.

  • Other modes of catabolism – which are really relatively "special" – do not apply to our oxygen relationship categories even if they may allow for anaerobic growth; these are anaerobic respiration (Exp. 7), anoxygenic phototrophy (Exp. 11B) and oxygenic phototrophy (not studied in this course).

  • So, if you're going to do a comprehensive description of any organism – including those way beyond the scope of this course – you should indicate what of the five catabolic processes it can perform. Notice (for example) how this is done on the handout for Experiment 14.

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Tuesday, April 2, 2013
About samples and the schedule.

The revised schedule (indicating the postponed experiments and the official final exam time) is posted here, and a hard copy (to supercede the old one) will be passed out in lab this week.

About the samples utilized in Experiments 11 and 15A:

  • For Experiment 11B, Period 2 (March 19, 20, 21): We finally had successful, blooming enrichments from which to streak Succinate Agar plates for isolated colonies. You would have used an enrichment inoculated from one of the following water samples:
    • Alliant Energy Pond – collected 3/4/13, enrichments made in class that week.
    • Yahara A – collected 3/12/13, enrichment made by JL on 3/12/13.
    • Yahara B – collected 3/12/13, enrichment made by JL on 3/12/13.
    • Yahara C – collected 3/12/13, enrichment made by JL on 3/12/13.
    With a wet mount of any of the samples (except Yahara C) we didn't see any unusual cell types. With Yahara C, there were a lot of spirilla among the usual short rods. The "Yahara" samples were collected from the Yahara River in the East Side of Madison.

  • We saw that the snow samples collected on 3/5/13 (outside of MSB) did not result in successful enrichments for Experiment 11B. (Section 001 had used an early snow sample, collected in the morning; the other sections used a later sample, collected in the evening.)

  • For the direct, dilution platings made for Experiments 11A and 11C, we had available the following samples (unless you brought in your own which is encouraged by the lab manual and always a better alternative):
    • Steenbock Planter No. 4 – collected 3/11/13.
    • Steenbock Planter No. 5 – collected 3/11/13.
    (These are arbitrary numbers assigned by JL.)

  • For the water sample in Experiment 15A, the Alliant Energy Pond sample (above) was taken out of the refrigerator and used directly.

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Monday, April 1, 2013
More things on-line.

I have just posted a "rough draft" of this week's lab lecture here. I will also briefly go over the take-home assignment (handed back this week) and say a few words about Virtual Experiments 10 and 11D.

We will be starting another unknown this week with Experiment 14A; the associated handout is posted here, and there will be better time to go over this experiment later. Remember that unknowns are not done in pairs or teams, and each persion is given a particular one to work with. This exercise will be hassle-free if you streak well today to achieve isolated colonies from which you can pick pure cultures next week! The three-phase method is always preferred; see Appendix B.

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Monday, April 1, 2013
A couple demonstrations related to Exp. 11B.

A note about the first video: This is the monitor of the demonstration microscope in the back of the lab which we have all seen already. The cells are constantly drifting in and out of focus, but the movement of the cells (especially the big one which is usually in the very center) can be seen reasonably well. This organism is our first view of the "spirillum" type of morphology this semester. Note that the video may take a little while to load completely.

In case our in-lab demonstration on phototaxis doesn't work out this semester (and even if it does), here is a video of what happens to the purple non-sulfur bacterium Rhodospirillum rubrum when its light source is cut off for a half-second. The cells respond by reversing the direction of their movement, as if they think they're getting into a dark environment and need to get back into the light. Remember the organisms we are after in Experiment 11B are anoxygenic phototrophs (as discussed before break) which is how they obtain electrons (and energy) when growing anaerobically in the light. They are actually "hard-wired" to be phototactic even when they are growing as aerobic respirers and do not require light! (Recall that respiration is a chemotrophic process.)

A short video I made a few semesters ago is posted here. It shows gas escaping out of a tube which contains a culture of purple non-sulfur photosynthetic bacteria that had been incubated in the light for 8-10 days. Hear the "pop" when the gas hits the Bunsen burner flame? That's how one can confirm hydrogen production in such tubes. Hopefully the tubes we inoculated today and put in the light will show such H2 production. As you may learn elsewhere, these organisms are of interest in the field of alternative energy production.

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Friday, March 29, 2013
Regarding the second quiz.

The upcoming quiz will only cover Experiments 8, 9, 10, 11 and 15 (up through the week after Spring Break). The answers to the remaining old quiz questions (posted earlier) are now here. Not all of the questions will be relevant for the quiz, but they can be helpful for the final and also in thinking about the Formal Lab Report (summarized in Appendix I). We can review Virtual Experiment 10 this coming week in lab.

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Monday, March 18, 2013
Regarding Dilution Problem in Take-Home #2.

To clarify what we mean by "even-numbered dilutions": We mean even-numbered exponents. So, what dilution do we make when we add 1 ml to 99 ml? Also, are you limited to only inoculating 1 ml or 0.1 ml into any plate? OK – enough hints! :-)

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Monday, March 18, 2013

A reminder that take-home assignments are not to be handed in as a group project, and this includes the Growth Curve as well as Unknowns and other items. Growth curves handed in with two or more names cannot be graded as those of others who handed theirs in individually, and you can still hand in an individual growth curve (with calculations) on your own as soon as you can.

I'll post Lab Lecture Notes for this week (no. 9) as soon as I can. This week I plan on finally discussing the Lab Report which is due near the end of the semester as well as clarifying some further general points about catabolism as we get more deeply into the experiment on the enrichment and isolation of the purple non-sulfur photosynthetic bacteria. Our basic summaries of catabolic processes are on-line: general overview (which you have seen before) and chemotrophy vs. phototrophy.

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Thursday, March 14, 2013
 Regarding the Virtual Labs.

SURPRISE! The Department's web server is up and running again, and the format of the virtual experiments looks better than ever. The old links are restored in the links box, and I don't have to reconstruct any of the experiments as was my plan. I will have to make copies of each one for future emergencies and revisions.

A big thank you to those who have been expressing great interest in the virtual lab experiments. I'm expecting all the items on the 2nd page of our Syllabus will be fulfilled successfully.

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Wednesday, March 13, 2013
Regarding the Take-Home Exercise No. 2.

The Take-Home Exercise No. 2 will be put on-line about mid-afternoon tomorrow – Thursday, March 14. The link will be in the box on the right. Like No. 1, it will be due at the beginning of lab next week.

I will be here all day Friday and Monday (at least from 9 to 4) if downloading is difficult and you would like to obtain a hard copy. My office is 2521 on the Linden Drive side of the second floor.

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Monday, March 11, 2013
Regarding a couple schedule changes.

I will post a revised schedule soon, but here's some information about this week:

  • Exp. 11B Period 2: Seeing how the enrichments for photosynthetic bacteria (which we set up in Period 1 last week) will probably take longer than a week to come up, we should postpone Period 2 of Exp. 11B until next week. Subsequent periods will therefore each happen a week later.

  • Exp. 9A Period 1: Remember this was originally scheduled for last week but postponed because of the quiz. I was thinking of rescheduling it for this week, but we have enough dilution plating to do as it is, so after Spring Break would be best.

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Monday, March 11, 2013

Be sure to check over your Growth Curve assignment before handing it in this week. For full credit you must indicate the units for your growth curve calculations (round off to just one decimal place) and other things noted in the box on page 24B. Again, notice the example on page 24 (lower right) and example calculations on the next page, but remember those example figures do not apply to our experiment! There are also additional reminders on the data sheet which we have on-line if you don't have yours.

I will have this week's lab lecture notes (period 8; at least in rough form) on-line later today. There are a number of things to consider for Exp. 11 and the associated report which isn't due until March 3.

I will pass back the graded quiz this week at the beginning of lab. Remember the definition of strain which we went over in each lab section, as it is an important concept in bacteriology and also biology in general. We used the orange strain of E. coli (from Exp. 5C) as an example of a strain showing a different set of characteristics from "normal" strains. The definition of "strain" was also part of the required reading (Appendix H). At least we can define "strain" as a pure culture that we know something about, and it doesn't even have to be identified to genus or species.

A lot of times we hear people mention the "O157:H7 strain" of E. coli. That is not a strain designation but rather a taxonomic subdivision of E. coli known as a serotype (or serovar), and we will learn more about that in Experiment 14.

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Thursday, March 7, 2013

Notes for this week's very short "lab lecture" are posted here.

For when you get to them and work them through, the solutions to the problems in Virtual Experiment 8B are on-line here. Just a couple examples are given to help to explain the concepts and calculations of mutation frequency and recombination frequency. Our old Experiment 8 handout gives a lab-lecture's worth of explanatory material for what we are doing in both 8A and 8B.

Regarding the growth curve which is due next week: If you go here and click on the graph in the upper right, you can download a sheet of 3-cycle semilog paper (if you didn't pick up any paper in lab). You would need two sheets, as our data requires at least four cycles. That web page also discusses semilog paper in general. Our graph is formatted like the example on page 24 in the manual (the one on the bottom right), and the formulas to use are on the following page.

Take a look at Experiment 11 for next week. Note that you will be writing a report on your work in one of the three parts of Experiment 11 –  namely 11A, 11B and 11C. More about this next week!

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Tuesday, March 5, 2013

A reminder about our general "weather advisory": Click here. We also have the forecast and current conditions on the bottom of this page.

According to the schedule, we have a bit of lab work to do today as well as the quiz. However, we are delaying Experiment 9A (Quantitation of Bacteriophages) until some time after Spring Break.

Remember that the Growth Curve assignment isn't due until next week. Examples and formulas are given in Exp. 5C, and guidelines are in the box on the bottom of page 24B. Also, the handout providing the class data (and some additional reminders) is reproduced here.

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Thursday, February 28, 2013

A key for checking your Exp. 7A results page (page 34) is posted here.

  • A link to photos of the cultures is given below the table on that page as well as a link to some added information (with photos) about Alcaligenes faecalis. So far, we don't have a similar page for Bacillus sphaericus which is also generally non-reactive (and "boring") in most of the tests we ran. Unlike most Bacillus species we come across, B. sphaericus is negative in the amylase test.

  • Now you have a reliable data base to figure out a likely identification for your unknown which is due next week.

  • I never did mention anything about the Dichotomous Key on the front of this week's handout. That was meant to be useful to highlight some things to look for in the known cultures. There are many ways to arrange such a key based on the data we have collected, and we will probably touch on the subject of such keys later in the semester. It can be helpful in identifying your unknown as well.

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Monday, February 25, 2013
SECOND UPDATE

with Wed. morning addition in red

I plan on passing back the take-home exercise this week in lab. If for some reason yours was not turned in, please do so as soon as you get to lab. I need to go over them briefly before I get on with the lab lecture which I hope will be brief. (See the posted lab lecture notes for Period 6 in the box on the right.)

I wound up not grading the second question which reads as follows: "Does the term colony-forming unit only apply to bacterial quantitation? Briefly explain why or why not." I should have left the word "bacterial" out altogether. Colony-forming unit is not a quantitative term. Any colony you see on your plates arose initially from a colony-forming unit which was composed of one or more cells from the sample that had landed on that spot of the medium initially. Whether or not a colony is counted, it still arose from a CFU! If you haven't done so already, please read this. The same information is in the opening paragraphs of Experiment 1 which also indicates that endospores and reproductive spores are also bacterial cells and can qualify as contributing to (or being) a colony-forming unit. I saw an awful lot of replicated mention of other single-celled organisms which are not relevant to the course and sometimes far off-base.

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Monday, February 25, 2013

The finalized lab lecture notes for last week are posted, as is the "draft" of this week's lab lecture notes. Note (in the box on the right) that the answers to the old, sample quiz questions are already on-line.

About the upcoming quiz:

  • The format is as follows: (1) multiple true-false questions (like most of the review questions), (2) matching items, (3) short answers, and (4) a couple very general dilution problems. We can include definitions of terms in the matching and short answer sections.

  • The quiz concerns Experiments 1 through 7, and be sure to include the virtual labs and relevant appendices in your review – especially Appendix B, C and D. Appendix D is full of background material for things we have gone through in lab so far this semester. Pay special heed to the terms in bold-face. Don't worry about knowing any of page 105 for the coming quiz, as we have rarely asked questions about the sterilization of media or equipment on our quizzes and exams, and you are probably not seeing any in the sample quiz questions.

  • The posted review questions can be a guide as to how deeply we may go into things. By all means do not memorize trivia such as the reactions and appearances of specific species or any specific formulations of media.

  • We often ask questions in the quiz about aseptic technique. You should review the videos from time to time – both of which are linked from this page. Also by now, you should be familiar with the material on the microscope handout passed out the first day.

About Experiment 7A this week in lab:

  • I am hoping that we make a great effort to see the twelve known cultures that were set up last week. This is our traditional "mixer" where we travel around neighboring tables and see the results such that we can fill in page 34 as much as possible. It is most important that we see the positive and negative results for the various test media. Once filled out, page 34 serves as our "database" to identify the unknowns for Experiment 7A.

  • A key to help you "tune up" your Exp. 7A results page (page 34) which you are filling out will be posted late Thursday afternoon and it includes some photos of the tube and plate reactions. Then you will have good results to figure out a likely identification for your unknown which is due next week (not this week).

  • The Differential Media site explains (with color photos) some of the media we use in Experiments 6 and 7 – most notably Motility Medium and Starch Agar. With Starch Agar and the amylase test, we are introduced to the topic of extracellular enzymes.

  • In case you are wondering about our use of the term "strain," our official definition is given here. We used an unusual orange-pigmented strain of E. coli in the growth curve experiment – a photo of which is here. Otherwise, the cultures we use in our various experiments (including those where we identify unknowns such as 7A) tend to be typical strains of their species.

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Tuesday, February 19, 2013

The Virtual Lab Manual Website (where most of our Virtual Experiments reside) appears to be down tonight.  Dr. Tim Paustian – the editor and webmaster of the Virtual Lab Manual – will hopefully get it going again tomorrow morning. Stay tuned.

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Thursday, February 14, 2013

The first "Take-Home Exercise" is now on-line here and ready to print out. Be sure to read the box just under your name. It's due first thing in lab next week.

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Tuesday, February 12, 2013

The first "Take-Home Exercise" (worth 25 points according to the Syllabus) will be put on-line for downloading on Thursday afternoon. This is to be worked on outside of lab and turned in first thing at the next lab session. Do note the introductory directions at the top. Having done the practice problems in "Virtual Experiment 4B" will help considerably in doing the dilution problems which are a part of the exercise.

Looking ahead to next week:  A draft of the Lab Lecture Notes for Period 5 is posted here. Under the heading "Some General Things about Catabolism," the material on the indicated handout is found on-line here. We try to make catabolism simple and relevant to the organisms we are working on, and there will be no pathways to memorize as you might find in other courses, notably biochemistry.

Also looking ahead to next weekVirtual Experiments 5A and 5B will make a lot more sense after you have read through Appendix D. We will apply the concept of "oxygen relationships" in several lab experiments to come.

  • Virtual Experiment 5A on oxygen relationships: The experiment in the Bacteriology Department's Virtual Manual that we use for this ("5-3") had to be improved, and it's presently posted for our course here. You will notice on page 23 (in our manual) a table for results titled "Oxygen Relationships and Related Physiological Processes." And you will also notice an "extra" introduction to the concept in the manual – in addition to the one on-line! Take your time going through 5A; we will find it helpful when we go through Experiment 7 in lab and also some later experiments.

  • Virtual Experiment 5B:
    • For media-making, we go to Experiment "5-2" in the Department's Virtual Manual which is here. There are no results to record; just read through the procedure.
    • For the example of a requirement for a growth factor, we go to Experiment "5-4" in the Department's Virtual Manual which is here. The table for recording results is at the bottom of page 23. Hopefully soon (at least before the day of the quiz) we will go over the concept of siderophores in lab.

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Friday, February 8, 2013

I have posted this week's finalized Lab Lecture Notes; see the box on the right for Period 3. You can also click on no. 4 for what is coming up next week.

Here's a hint that may help with the use of the microscope: With your marker, put a mark near the smear that you are going to be looking at. This mark should be easy to focus on, and the cells will be nearby. When you switch to the oil immersion lens, you only need to move it up or down (with the fine adjustment) no more than 1/3 turn. Also, make sure that the 10X lens is clean; a good rub with oil-free lens paper works best.

If the concepts of colony-forming units and scientific notation are troublesome, click here for CFUs and here for scientific notation. Do not use Wikipedia for CFU as we explained in lab.

According to the schedule, Appendix H will be required reading soon, and it should be helpful regarding terminology (including scientific names).

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Thursday, February 7, 2013

In the box on the right, I have posted links to some review questions (mostly taken from quizzes in previous semesters) to give you an idea of the kinds of questions we may ask in the in-lab quizzes and also occasionally in the "Take-Home Exercises." The numbers refer to the experiment numbers in the manual and go all the way to Experiment 17. The first in-lab quiz will cover Experiments 1 through 7 plus the relevant material in the Appendices.

On a Take-Home Exercise given last semester, I asked for definitions of bacteria and colony-forming unit – a couple terms we have gone over already in lab. Unfortunately the common answer for colony-forming unit was the first sentence one comes to in the Wikipedia article on the subject. Wrong! That sentence obviously defines nothing, and later on in the article we get a better idea as to what the term is about. However, the term is not necessarily restricted to counting bacteria!

Stick with the manual and the material we are learning in lab – not something written by a relative amateur on Wikipedia.

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Wednesday, February 6, 2013

We have finally gotten the set date and time for the scheduled final for Microbiology 102, and it's a little earlier than what was mentioned in one or more sections last week. It is Wednesday, May 15 from 12:25 PM to 2:25 PM. If you were planning on taking the final according to on-line information which you may have previously picked up from the Registrar's Office, we can indeed honor that time. Also, as mentioned in the lab, we will have alternate times available for those who have exam time conflicts, too many exams in a short amount of time, etc. So, let's not worry about this till late April or so.

Also, I presently plan on being away from Friday night through Sunday night. If the weather up north is bad, I actually may not make it back here until Monday night. As mentioned many times already, I am e-mailable. Keep an eye on these updates anyway.

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Tuesday, February 5, 2013

We now have a lost and found cabinet in the lab. It's the top cabinet (with glass door) that is closest to the front door of the lab. I have just been informed that the Friday Clean-up Crew found an Epi-Pen. If it's yours, please claim it soon.

If you find you must miss a lab (due to sickness, interview, participation in an out-of-town athletic event, etc.), please note what we've written below about our attendance policy. Beyond that, you can e-mail me to let me know you'll be absent plus anything else.

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Monday, February 4, 2013

For the upcoming lab periods, always be sure to check the schedule well ahead of time for which lab experiments are coming up and also the things in the "items due" column. From time to time in these updates, I'll make note of certain associated items that need attention, but I won't always repeat what is happening or "due" on the schedule.

Regarding this week in lab:

  • Be sure to have read over Experiment 4A. Most of you have probably used pipettors in Chemistry to transfer specific amounts of solutions. We use them in our course to transfer suspensions aseptically. (Remember, bacteria are particles, and we could never consider a liquid containing bacteria or even viruses as being a solution.) As noted in Experiment 4A, we refer to page 95 in the appendix for some important points about the aseptic use of pipettors.

  • With Experiment 1 (Period 3) and also Exp. 4A, we are getting into the concept of Dilution Theory. Note how we inoculated the soil and water samples into the plates last week. This week, we will take into account (1) the number of colonies on these plates, (2) the amount of sample we inoculated (which was 1 ml), and (3) the dilution of that sample; these things will be factored together to tell us how much these samples are "contaminated" with bacteria. Looking ahead to Period 3, you can see how this is done. It should be pretty straightforward, and you have already considered making dilutions in Chemistry utilizing the same principles.

  • Appendix C explains the concept of dilution theory, and an easier-to-understand equivalent to this appendix which we have on the web is our first dilution plating page which is especially relevant to Exp. 1 (period 3) and also our second dilution plating page which goes along with Exp. 4A in which we will be determining the number of colony-forming units per gram of hamburger!

  • Note that the "due date" for Virtual Experiment 4B is next week. By then, dilution theory will make a lot more sense from what we are doing in lab this week. Again, for virtual experiments, there is not anything to hand in, and you can check your answers to the practice problems in Exp. 4B by following the link to the posted solutions.

I am sure that going through the videos again will make a lot more sense, considering how we did these techniques this week in lab. Two things to especially keep in mind: (1) not to let the caps and plugs lay on the table during the transfer of cultures and (2) never to have any tube open and upright in the test tube rack while making transfers. If the diagram on page 92 cannot be followed, it is perfectly OK to handle one tube at a time.

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Monday, February 4, 2013

A special announcement: The Kick-Off Meeting for VIDA (Volunteers for Intercultural and Definitive Adventures) meets Tuesday this week. Click on this.

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Tuesday, January 29, 2013

For most lab sessions throughout the semester, I will be passing out a handout showing a list of the materials (media, cultures, samples) with their color key as we can't mark each item individually as to what it is. The one for this week is here.  For the Tuesday lab which already met today, there is an error: It is Virtual Experiment 3B (not 4B) that we consider doing for next week.

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Monday, January 28, 2013

As pointed out in our first lab meeting, there are a number of things to read over for this week's lab:

  • Be sure to have read over the introductions to Experiments 1, 2 and 3. Also Appendix B, and the Gram Stain on page 117.

  • Also look at the Aseptic Technique items under "Microbiological Concepts" in the list of links to the right – specifically those that are associated with videos: Tube-to-tube transfers and three-phase plate-streaking. We will be doing these techniques next week and throughout the semester.

  • It will also help to read the handouts which were passed out this week.

If you were unable to get the lab manual in time for this week's lab, the relevant pages are here. There are eight downloadable pages.

Looking ahead to Experiment 3B – our first virtual lab – it would help to first go through our "Introduction to the Virtual Lab Experience" which is here. Then, for Exp. 3B, click here and scroll down to Period 4 in the experiment which is part of the Department's on-line general lab manual. In Period 4, there is a misprint regarding the directions for the acid-fast stain; it's actually on page 119.

For virtual experiments in general, assume that the procedures have been done, and you are recording the results for which there is space in the manual. There will be nothing to hand in, although the material can show up on quizzes and the final.

These days I tend to be at work here Sunday thru Thursday rather than Monday thru Friday. Except for brief periods on Fridays and Saturdays when I am in the land of limited internet access, I can access emails OK.

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Tuesday, January 15, 2013

Welcome to the Microbiology 102 Website where we find it easy to post course announcements and links to relevant material on the web. These things are equivalent to Course News and Content found on Learn@UW. Eventually we will utilize Learn@UW for posting grades.

Information on obtaining the Lab Manual and other supplies for the semester is on our course syllabus which is posted here. On our first day in lab, we will be passing out the hard copies of the Syllabus and Schedule. If you have not obtained the manual for doing the lab work on the first or second period, we will supply a handout with the necessary information. This material is already on-line and linked from the box on the right so that you can look it over early. You will find that the Lab Manual (which is usually updated for each semester) will be our primary source of essential information for Microbiology 102.

Going along with the Syllabus, here are some good things to know:

  • Our attendance policy:  We expect you to show up for every lab period, as your lab partner and your observations and understanding of the results and concepts of your experiments depend on it. Let us know if you cannot attend lab for sickness or any other reason. If you do miss a lab, please heed the following before e-mailing your instructor with questions about what material was missed and what to do about making it up: Consult the schedule and the manual regarding the lab material that was missed, and make sure that you see the results of the procedures that were done in your absence when you get back. We will often repeat important procedures in a coming lab period. We can save the things you inoculated and also afford the opportunity to start any "unknown" you missed getting on the day you were absent. And note that the Lab Lecture Notes will always be posted on-line (sooner or later, hopefully sooner).

    Two unexplained absences in a row will tell us that you have dropped the course.

  • Getting your two credits-worth:  Like any course, it is necessary to keep up with things right along. Before we had to convert to the present system where a number of laboratory experiments were converted to virtual exercises, the lab met the usual four hours a week during a regular semester for the two credits. Now, the course only meets once a week (for two hours) for the in-lab experiments. So – outside of the lab – you should plan on spending a good amount of time
    1. reviewing things,
    2. looking ahead to the next lab period (according to the schedule), and
    3. working through the virtual experiments.
    We will be explaining more about what is expected for the virtual experiments in the coming weeks.

    Our lab presentations (opening lecture with good stuff on the whiteboard), the on-line lecture notes (to "tune up" yours), and the updates and links (on this page) should help to amplify the schedule and clarify the subject matter. Hopefully, the experiments we do and their results should make sense – including what they mean in light of the "big picture" which we hope to develop as the semester continues.

  • Our Bad Weather Policy for Microbiology 102:  In case of inclement weather, especially when the roads are clogged and buses won't run, you should USE YOUR BEST JUDGEMENT about coming in or not. Heavy snow and icy roads and sidewalks can make getting around difficult or impossible. Unless the University publicizes the fact that it is closing down due to bad weather conditions, our course is still on, but do not feel that you must come in if it presents a possible danger. See our link to local weather conditions and forecast below.

This page is the homepage of the
Microbiology 102 website.
Last updated on 5/20/13
at 12:45 PM, CDT
.

John Lindquist: homepage,
UW e-mail and g-mail.

Department of Bacteriology,
University of Wisconsin-Madison.

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