CATABOLISM
The Opposite of Biosynthesis (Anabolism)

AN ULTRA-SIMPLIFIED GENERAL REVIEW
leaving out enzymes and intermediate steps to a great
extent in order to examine the basic transfer of electrons.


Purposes of Catabolism:

  1. To release energy which can be used to generate ATP from ADP and phosphate.

  2. To release electrons which serve as "reducing power."

  3. Also to provide some of the "building blocks" for biosynthesis.

Regarding their source of reducing power, organisms can be classified as either organotrophs or lithotrophs:

  1. ORGANOTROPHS oxidize organic compounds. As an example, the complete oxidation of a typical carbohydrate (represented by CH2O) by respiration by an organotroph can be represented as:
    CH2O + O2 --> CO2 + H2O.

  2. LITHOTROPHS oxidize inorganic compounds. Examples of electron donors that are oxidized include:
    • H2 (hydrogen gas), oxidized to H2O
    • S (sulfide), oxidized to SO42– (sulfate)
    • Fe2+ (ferrous ion), oxidized to Fe3+ (ferric ion)
    • NH4+ (ammonium), oxidized to NO2 (nitrite)
    • NO2 (nitrite), oxidized to NO3 (nitrate)

Regarding their ultimate source of energy, organisms can be classified as either chemotrophs or phototrophs:

CHEMOTROPHS
utilize chemical reactions in the generation of energy (and also reducing power). This is done by fermentation and/or respiration. In the general microbiology lab, the ability of a chemoorganotrophic organism to respire (with oxygen) and/or ferment can be determined with the "oxygen relationship test." A review of respiration vs. fermentation is given here.

PHOTOTROPHS
also oxidize chemical compounds, but the ultimate source of energy is light. "Phototrophy" is shown here as the set of light-dependant, catabolic reactions associated with photosynthesis (the other reactions of which are anabolic).

• FERMENTATION:

To make a long story short, we can use the example of a typical fermentation pathway (stepwise/sequential chain of chemical reactions) where glucose is oxidized to pyruvate (the oxidation stage of the pathway) and pyruvate is then reduced to fermentation products such as lactic acid (the reduction stage of the pathway). At a certain step in the oxidation stage where compound A loses electrons (which are picked up by co-enzyme NAD+), the electrons can be transferred to compound C in the reduction stage (thus "regenerating" the NAD+).

In the generation of ATP, the "P" (phosphate) can be "free" (inorganic) or attached to an intermediate compound in the pathway (organic). As this phosphate is transferred to ADP in the formation of ATP and does not incorporate energy associated with electron transport, the term "substrate-level phosphorylation" applies.

• PHOTOTROPHY:


("chl" represents chlorophyll.)

Phototrophy can be OXYGENIC (evolving O2 when H2O serves as the electron donor) or ANOXYGENIC (non-O2-evolving). Note in these pages that cyclic vs. non-cyclic photophosphorylation is not (yet) addressed.

 
 
 
 
 

• RESPIRATION:

While fermenters tend to be organotrophs, respirers can be organotrophs or lithotrophs. For respiration, we have O2 as an external electron acceptor, and the reduction stage seen in fermentation is replaced here by continued oxidation. An organic substrate can be oxidized completely to CO2 as O2 is reduced to H2O.

ANAEROBIC RESPIRATION employs an electron acceptor other than O2 such as nitrate (NO3) which is reduced to nitrite (NO2) or N2.


An attempt to produce an abbreviated summary of catabolism based on the above follows.
Here we get down to the basics, leaving out cytochromes and other intermediates. This diagram represents what generally happens at a step in the catabolic pathway where a substrate is oxidized with (1) electrons given off which can be utilized for "reducing power" and (2) energy is released that results in the generation of ATP. For fermentation (as noted above), ATP generation is accomplished by substrate-level phosphorylation rather than with energy released at such a step in the pathway.


The above basic flow diagram can be expanded to address the concept of "reducing power," the provision of electrons and ATP to anabolic activities, and the key role of NAD:


Combining Terms: Using the terms describing organisms according to their source of energy and reducing power as defined above, one can combine these terms as follows: chemoorganotroph, chemolithotroph, photoorganotroph, and photolithotroph. Considering the terminology associated with the source of carbon as explained here, one finds that most chemoorganotrophs are chemoheterotrophs (and vice versa), and most chemolithotrophs are chemoautotrophs (and vice versa). With this in mind, the following terms are paired accordingly: photoorganotrophs with photoheterotrophs, and photolithotrophs with photoautotrophs.

Reasons why an organism may grow anaerobically are summarized here.


A page on the Bact. 102 website summarizing catabolism and the test for oxygen relationships can be found here.



GO 
TO:
Selected General Microbiology Topics:
 Bacterial Nutrition and Growth
 Review of the Cycles of Elements
 Differential Media Site
Archived Bacteriology 102 Site
Site Outline of related pages

These general microbiology pages have copyright by John Lindquist
and found their permanent sanctuary on www.jlindquist.net circa 2001.
Copies found elsewhere are neither authorized nor up to date.
This page was last tuned up on 10/15/07 at 10:45 AM, CDT.
John Lindquist, Department of Bacteriology,
University of Wisconsin – Madison